The Link to X-Ray Protein Crystallography


A. Crystal Production

(wet lab)

  1. Production
  2. Purification
  3. Crystallisation

 

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B. Data Collection

(x-ray lab)

  1. Mounting
  2. Shooting
  3. Detection

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C. Structure Solution

(computer lab)

  1. Indexing
  2. Integration
  3. Scaling
  4. Phasing
  5. Building
  6. Refinement
  7. Validation
  8. Publication

Building

The result of the correct assignement of the structure factors is an electron density map. An atomic model of the structure has to be built into the electron density map. The correct building of the structure is used during refinement to improve the (approximate) phases obtained earlier.

LINKS

CIMR Protein Crystallography Course

PROGRAMMES

COOT building, refinement
O building
Quanta building (commercial software)
ARP/wARP automatic building, refinement
Buccaneer automatic building
RESOLVE automatic building, density modification

Mission / Organisations / Trivia / Links / Florian Fisch / 5 June 200