The Link to X-Ray Protein Crystallography

A. Crystal Production

(wet lab)

  1. Production
  2. Purification
  3. Crystallisation

 
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B. Data Collection

(x-ray lab)

  1. Mounting
  2. Shooting
  3. Detection

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C. Structure Solution

(computer lab)

  1. Indexing
  2. Integration
  3. Scaling
  4. Phasing
  5. Building
  6. Refinement
  7. Validation
  8. Publication

Integration

The intensities of the reflections are the actual experimental data of a crystallography experiment. Therefore integration is a crucial step during the data processing. An intensity value (I) and a background value (σI) are determined and saved. Several errors can occur and have to be accounted for. Most of the reflections can be hidden in the background, the signal can be saturated and reflections can be hidden by the backstop of the detector. Wrong values are generally excluded from the analysis. This is generally automatically done by the integration programmes.

PROGRAMMES

MOSFLM, indexing, integration
HKL2000, indexing, integration

Mission / Organisations / Trivia / Links / Florian Fisch / 18 July 2009