The Link to X-Ray Protein Crystallography

A. Crystal Production

(wet lab)

  1. Production
  2. Purification
  3. Crystallisation

 
>

B. Data Collection

(x-ray lab)

  1. Mounting
  2. Shooting
  3. Detection

>

C. Structure Solution

(computer lab)

  1. Indexing
  2. Integration
  3. Scaling
  4. Phasing
  5. Building
  6. Refinement
  7. Validation
  8. Publication

Publication

Protein structures are generally deposited in the protein database as scientific journals generally require. There are two parts of information: the atomic coordinates and the electron density map. Other databases classify proteins in different ways. For graphical presentation there is a whole series of programmes available.

DATABASES

wwPDB atomic coordinates, cosortium of RSCB PDB, PDBe, PDBj
EDS electron density maps
CATH structure classification
SCOP structure classification

PROGRAMMES

CCP4MG visualise and present data
PYMOL visualise data
Jmol visualise data

Mission / Organisations / Trivia / Links / Florian Fisch / 5 June 2009